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S49.1G.3

規格:

價格:

編號:B165650

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

S49.1G.3

商品貨號

B165650

Organism

Mus musculus, mouse

Cell Type

T lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

lymphoma

Strain

BALB/c

Applications

The cells are sensitive to 0.01 mM thymidine, 0.001 mM hydrocortisone and 5 mcg/ml phytohemagglutinin.

Tested and found negative for ectromelia virus (mousepox).

Antigen Expression

H-2d; Thy-1.2; TL

Genes Expressed

H-2d; Thy-1.2; TL

Comments

This variant of S49.1 (ATCC TIB-28) was selected for resistance to 1 mM 6-thioguanine.

The cells are sensitive to 0.01 mM thymidine, 0.001 mM hydrocortisone and 5 mcg/ml phytohemagglutinin.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium

Dulbecco's modified Eagle's medium, 90%; horse serum, 10%

Subculturing

Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Name of Depositor

A Harris

Deposited As

Mus musculus

References

Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928

B165651：S49.1G.3 PHA.100/0 (replaces S49.1G3 PHA.12P)

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