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PAb 108
PAb 108
規(guī)格:
價格:
編號:B165478
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
PAb 108
商品貨號
B165478
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody cross-reacts with the large T antigen of BK virus.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a determinant on the N terminal region of large T antigen and small t antigen (SV40)
Cellular Products
immunoglobulin; monoclonal antibody; against a determinant on the N terminal region of large T antigen and small t antigen (SV40)
Comments
Animals were immunized with SV40 transformed BALB/3T3 cells.
Spleen cells were fused with NS-1 myeloma cells.
The antibody cross-reacts with the large T antigen of BK virus.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Dulbecco's modified Eagle's medium, 80%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2a
Name of Depositor
EG Gurney
Deposited As
mouse (B cell); mouse (myeloma)
References

Gurney EG, et al. Antigenic binding sites of monoclonal antibodies specific for simian virus 40 large T antigen. J. Virol. 57: 1168-1172, 1986. PubMed: 2419584

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