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pAB366Ben

規格:

價格:

編號:B233285

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pAB366Ben

商品貨號

B233285

Designations

pAB366Ben

Depositors

BM Tyler

培養基

Biosafety Level

Vector Information

Size (kb): 7.1999998092651370

Vector: pAB366Ben (plasmid)

Construction: pAB366, pBT6

Marker(s):ampR,bleR,benR

Construct size (kb): 7.199999809265137

Features: marker(s): ampR, bleR, benR
replicon: pMB1

Applications

contains sequence tubulin, beta

in another host, produces protein tubulin, beta

reporter construct

shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--5.1, 2.1; HindIII--4.0, 3.2; BamHI/HindIII--2.6, 2.5, 1.5, 0.65; BamHI/XhoI--3.0, 1.35, 1.2, 0.9, 0.8; SalI/HindIII--3.3, 2.6, 0.85, 0.55.

A 2.5 kb SalI/HindIII fragment contains the complete tubulin gene and 5' and 3' flanking regions.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Austin B, et al. Optimized vectors and selection for transformation of Neurospora crassa and Aspergillus nidulans to bleomycin and phleomycin resistance. Gene 93: 157-162, 1990. PubMed: 1699844

Orbach MJ, et al. Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker. Mol. Cell. Biol. 6: 2452-2461, 1986. PubMed: 2946938

Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151

Shipped

frozen

Shipping Information

?Frozen glycerol stock of Escherichia coli containing the plasmid.

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