Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Wild-type Jurkat cells were mutagenized with the frameshifting mutagen ICR-191 and clones were isolated that failed to increase calcium in response to pervanadate.Ref
Williams BL, et al. Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor: reconstitution studies in a ZAP-70-deficient Jurkat T- cell line. Mol. Cell. Biol. 18: 1388-1399, 1998. PubMed: 9488454
The P116 cell line displays severe defects in TCR-induced signaling functions, including protein tyrosine phosphorylation, intracellular calcium mobilization, and interleukin-2 (IL-2) promoter-driven transcription. These signaling defects can be fully reversed by reintroduction of catalytically active versions of either Syk or ZAP-70 into the P116 cells.RefWilliams BL, et al. Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor: reconstitution studies in a ZAP-70-deficient Jurkat T- cell line. Mol. Cell. Biol. 18: 1388-1399, 1998. PubMed: 9488454
The cell line expresses no detectable ZAP-70 mRNA or protein and also fails to express Syk. RefWilliams BL, et al. Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor: reconstitution studies in a ZAP-70-deficient Jurkat T- cell line. Mol. Cell. Biol. 18: 1388-1399, 1998. PubMed: 9488454
The P116 cell line is a ZAP-70 deficient mutant of the E6-1 clone of Jurkat (ATCC TIB-152). RefWilliams BL, et al. Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor: reconstitution studies in a ZAP-70-deficient Jurkat T- cell line. Mol. Cell. Biol. 18: 1388-1399, 1998. PubMed: 9488454
The P116.cl39 derivative (ATCC CRL-2677) stably expresses the ZAP-70 kinase.
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 2 to 3 x 106 viable cells/mL. Cells can reach a density of 6 x 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Williams BL, et al. Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor: reconstitution studies in a ZAP-70-deficient Jurkat T- cell line. Mol. Cell. Biol. 18: 1388-1399, 1998. PubMed: 9488454
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
