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P116.cl39 [P116.c39]
P116.cl39 [P116.c39]
規格:
價格:
編號:B165449
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
P116.cl39 [P116.c39]
商品貨號
B165449
Organism
Homo sapiens, human
Cell Type
T lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension, multicell aggregates
Biosafety Level
2  [Cells contain CMV and SV-40 viral DNA sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
acute T cell leukemia
Gender
male
Applications
The P116.cl39 cell line was derived from the ZAP-70 deficient P116 cell line (ATCC CRL-2676) by transfection with a wild-type ZAP-70 expression vector, pcDNA3-mZAP.
P116.cl39 cells stably expresses the ZAP-70 kinase.
The cell line can be used as a control for the ZAP-70 negative P116 cells.
Storage Conditions
liquid nitrogen vapor phase
Derivation
The P116.cl39 cell line was derived from the ZAP-70 deficient P116 cell line (ATCC CRL-2676) by transfection with a wild-type ZAP-70 expression vector, pcDNA3-mZAP.
Clinical Data
male
Comments
The P116.cl39 cell line was derived from the ZAP-70 deficient P116 cell line (ATCC CRL-2676) by transfection with a wild-type ZAP-70 expression vector, pcDNA3-mZAP.
The vector contains cytomegalovirus (CMV) and SV40 viral DNA sequences and the neomycin resistance gene.
P116.cl39 cells stably expresses the ZAP-70 kinase. The cell line can be used as a control for the ZAP-70 negative P116 cells.
Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.4 mg/ml G418, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 2 to 3 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor
RT Abraham
Deposited As
human
References

Williams BL, et al. Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor: reconstitution studies in a ZAP-70-deficient Jurkat T- cell line. Mol. Cell. Biol. 18: 1388-1399, 1998. PubMed: 9488454

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