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MJ [G11]
MJ [G11]
規格:
價格:
編號:B165160
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MJ [G11]
商品貨號
B165160
Organism
Homo sapiens, human
Tissue
peripheral blood
培養基
Cell Type
T lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2  [Cells contain Retrovirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
cutaneous T cell lymphoma; mycosis fungoides
Age
50 years
Gender
male
Ethnicity
Caucasian
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
This line was established by Popovic et al. in 1983 from peripheral blood of a patient with CTCL.
Clinical Data
male
50 years
Caucasian

Antigen Expression
CD2 +; CD3 +; CD4 +
Receptor Expression
interleukin 2 (IL-2)
Genes Expressed
HTLV-I,CD2 +; CD3 +; CD4 +
Cellular Products
HTLV-I
Comments
The cells produce Human T cell Leukemia virus (HTLV-I) and HTLV related antigens.

The cells express a cell surface glycoprotein of 61000 daltons (45000 dalton when not glycosylated) that is characteristic of HTLV infection.


Complete Growth Medium
Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Subculturing
Pellet cells by centrifugation, save one third of the spent medium and to two volumes of fresh medium. Resuspend the cell pellet in the mixture and dispense into new flasks. Establish new cultures at about 1 X 106 cells/mL.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12,14
D16S539: 11,12
D5S818: 11,13
D7S820: 8,11
THO1: 6,7
TPOX: 8,11
vWA: 15,17
Name of Depositor
President and Fellows of Harvard College
Deposited As
Homo sapiens
U.S. Patent Number
Year of Origin
1983
References

Essex ME, Lee TH. Method and products for detection of human T cell leukemia virus. US Patent 4,743,678 dated May 10 1988

Popovic M, et al. Isolation and transmission of human retrovirus (human t-cell leukemia virus). Science 219: 856-859, 1983. PubMed: 6600519

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