午夜性色福利视频,成人免费视频网站,久久三区

PMJ2-R

規(guī)格:

價(jià)格:

編號:B165517

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產(chǎn)品名稱

PMJ2-R

商品貨號

B165517

Organism

Mus musculus, mouse

Cell Type

peritoneal macrophage; infected with J2 viru

培養(yǎng)基

Product Format

frozen

Morphology

macrophage

Culture Properties

suspension; some adherent cells

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Gender

female

Strain

C57BL/6J

Applications

Cell staining demonstrated intracellular expression of the product of the v-raf gene in these cell lines.

Analysis demonstrated the presence of peritoneal macrophage associated cell surface antigens, interleukin-6 (IL-6) secretion induced by lipopolysaccharide (LPS), and biological response modifier-induced cytotoxic activity against tumor cells.

The cells are phagocytic, non-specific esterase positive and are Fc receptor positive.

Storage Conditions

liquid nitrogen vapor phase

Clinical Data

female

Antigen Expression

MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; F4/80 +; Ly-5 +; Lyt-1.1 -; Lyt-1.2 -; Thy-1.2 -; sIg -

Receptor Expression

Fc receptor (FcR)

Genes Expressed

MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; F4/80 +; Ly-5 +; Lyt-1.1 -; Lyt-1.2 -; Thy-1.2 -; sIg -

Comments

PMJ2-PC (ATCC CRL-2457) and AMJ2-R (ATCC CRL-2458) are cloned, continuous, peritoneal macrophage cell lines generated from C57BL6J mice by in vivo infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes.

Cell staining demonstrated intracellular expression of the product of the v-raf gene in these cell lines.

The in vivo immortalized cells had many of the morphological and functional characteristics of peritoneal macrophages.

The cells are phagocytic, non-specific esterase positive and are Fc receptor positive.

One of the clones, PMJ2-PC, constitutively expressed major histocompatibility complex (MHC) class II antigens, and in the other clone, PMJ2-R, MHC class II antigens expression was induced by recombinant murine interferon-gamma.

Complete Growth Medium

Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 5 mM HEPES, 95%; fetal bovine serum, 5%

Subculturing

Scrape off the attached cells and transfer along with the floating cells into new flasks.

Medium Renewal: Twice per week

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions

Temperature: 37°C

Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%

Name of Depositor

AV Palleroni

Deposited As

mouse

References

Adami C, et al. In vivo immortalization of murine peritoneal macrophages: a new rapid and efficient method for obtaining macrophage cell lines. J. Leukocyte Biol. 53: 475-478, 1993. PubMed: 7683328

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

王經(jīng)理

手機(jī)號：17280875617

QQ：1438578920
胡經(jīng)理

手機(jī)號：13345964880

QQ：2438244627
周經(jīng)理

手機(jī)號：17757487661

QQ：1296385441
于經(jīng)理

手機(jī)號：18067160830

QQ：2088210172
沈經(jīng)理

手機(jī)號：19548299266

QQ：2662369050
李經(jīng)理

手機(jī)號：13626845108

QQ：972239479