Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody is specific for polypyrimidine tract binding protein (PTB).
The antibody may be used in immunodepletion experiments.
Derivation
Spleen cells were fused with Sp2/0 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against polypyrimidine tract binding protein (PTB)
Cellular Products
immunoglobulin; monoclonal antibody; against polypyrimidine tract binding protein (PTB)
Comments
Animals were immunized with bacterially expressed full length GST-PTB recombinant protein.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
The antibody is specific for polypyrimidine tract binding protein (PTB).
Polypyrimidine tract binding protein (PTB) is a represssor of pre-mRNA splicing.
The antibody may be used in immunodepletion experiments.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 10 mM HEPES, 0.1 mM nonessential amino acids and 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
