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mAb64
mAb64
規格:
價格:
編號:B165073
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
mAb64
商品貨號
B165073
Organism
Rattus norvegicus (B cell); Rattus norvegicus (myeloma), rat (B cell); rat (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? Lou (B cell); Lou (myeloma)

Applications
The antibody is specific for the alpha subunit of AChR and does not react with the main immunogenic region of AChR.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized subcutaneously with purified, intact bovine calf acetylcholine receptor.
Spleen cells were fused with Y3 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against acetylcholine receptor (AChR) alpha subunit
Cellular Products
immunoglobulin; monoclonal antibody; against acetylcholine receptor (AChR) alpha subunit
Comments
Animals were immunized subcutaneously with purified, intact bovine calf acetylcholine receptor.
Spleen cells were fused with Y3 myeloma cells.
The antibody is specific for the alpha subunit of AChR and does not react with the main immunogenic region of AChR.
The antibody a has low affinity for S. aureus protein A.
Complete Growth Medium
Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.01 mg/ml insulin, 0.01 mg/ml transferrin, 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2a
Name of Depositor
Salk Institute for Biological Studies
Deposited As
rat (B cell); rat (myeloma)
U.S. Patent Number
References

Lindstrom JM. Assays for myasthenia gravis. US Patent 4,789,640 dated Dec 6 1988

Tzartos S, et al. Characteristics of monoclonal antibodies to denatured Torpedo and to native calf acetylcholine receptors: species, subunit and region specificity. J. Neuroimmunol. 10: 235-253, 1986. PubMed: 3484485

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