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Mab 543

規(guī)格:

價(jià)格:

編號(hào):B165069

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

Mab 543

商品貨號(hào)

B165069

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養(yǎng)基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications

The antibody binds to a site distinct from the C3b binding site, and can be used to measure the number of receptors despite occupancy by ligand.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Animals were immunized with purified CR1 from human erythrocytes.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against CR1 (human complement C3b receptor, CD35)

Cellular Products

immunoglobulin; monoclonal antibody; against CR1 (human complement C3b receptor, CD35)

Tumorigenic

Yes

Effects

Yes, forms ascites in BALB/c mice

Comments

Animals were immunized with purified CR1 from human erythrocytes.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody binds to a site distinct from the C3b binding site, and can be used to measure the number of receptors despite occupancy by ligand.

Complete Growth Medium

The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
0.05 mM 2-Mercaptoethanol

Subculturing

Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.

Cryopreservation

culture medium 95%; DMSO, 5%

Culture Conditions

Temperature: 37.0°C

Isotype

IgG1; kappa light chain

Name of Depositor

Scripps Clinic and Research Foundation

Deposited As

mouse (B cell); mouse (myeloma)

U.S. Patent Number

4,672,044