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Mab 126
Mab 126
規格:
價格:
編號:B165062
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Mab 126
商品貨號
B165062
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Neuroblastoma,Neuroblastoma, Carcinoma,Adenocarcinoma
Applications
The antibody reacts strongly with neuroblastoma and melanoma cells, but does not react with lymphomas, sarcomas and most carcinomas.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against ganglioside GD2
Cellular Products
immunoglobulin; monoclonal antibody; against ganglioside GD2
Tumorigenic
Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with neuroblastoma cells (LAN-1).
Spleen cells were fused with P3X63Ag8 myeloma cells.
Ganglioside GD2 is strongly expressed on cells of tumors of neuroectodermal origin (especially neuroblastoma and melanoma cells).
The antibody reacts strongly with neuroblastoma and melanoma cells, but does not react with lymphomas, sarcomas and most carcinomas.
Faint reactivity was reported for small cell carcinomas, adenocarcinomas of the adrenal cortex, gliomas and normal neural and muscle tissue.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgM
Name of Depositor
Scripps Clinic and Research Foundation
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Reisfeld RA. Monoclonal antibody directed to human ganglioside GD2. US Patent 4,675,287 dated Jun 23 1987

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