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2E6
2E6
規格:
價格:
編號:B163683
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
2E6
商品貨號
B163683
Organism
Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)
Cell Type
hybridoma:lymphoblast B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts precipitates a 150000/90000 dalton CD11/CD18 heterodimer (see also ATCC HB-225) from murine leukocytes.
Storage Conditions
liquid nitrogen vapor temperature
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse beta 2 leukocyte integrins (CD18)
Cellular Products
immunoglobulin; monoclonal antibody; against mouse beta 2 leukocyte integrins (CD18)
Comments
Animals were immunized with mouse dendritic cells.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts precipitates a 150000/90000 dalton CD11/CD18 heterodimer (see also ATCC HB-225) from murine leukocytes.
2E6 blocks the binding function of LFA-1 (CD11a) and CR3/Mac-1 (CD11b).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 0.05 mM 2-mercaptoethanol

Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Isotype
IgG
Name of Depositor
RM Steinman
Deposited As
hamster, Armenian (B cell); mouse (myeloma)
References

Metlay JP, et al. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J. Exp. Med. 171: 1753-1771, 1990. PubMed: 2185332

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