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M-2E6
M-2E6
規格:
價格:
編號:B187668
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
M-2E6
商品貨號
B187668
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the Fab portion of human IgM.

Derivation
Animals were immunized with purified human IgM.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human IgM
Cellular Products
immunoglobulin; monoclonal antibody; against human IgM
Comments
The antibody reacts with the Fab portion of human IgM.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Isotype
IgG1
Name of Depositor
B Forghani, NJ Schmidt
Deposited As
mouse (B cell); mouse (myeloma)
References

Forghani B, et al. Use of monoclonal antibodies to human immunoglobulin M in "capture" assays for measles and rubella immunoglobulin M. J. Clin. Microbiol. 18: 652-657, 1983. PubMed: 6355153

Forghani B, et al. Production of monoclonal antibodies to human IgM for assay of viral IgM antibodies. J. Virol. Methods 5: 317-327, 1982. PubMed: 6819305

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