Cross-reacts with AAV-3; no hemagglutinin detected
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Depends upon helper virus Human adenovirus 2 ATCC® VR-846™ for replication. Helper virus may be inactivated at 56°C for 30 minutes.
Resistant to heat 56°C 4 hours; this property can be used to selectively inactivate the helper adenovirus. Resistant to ether and chloroform.
Cross-reacts with AAV-3; no hemagglutinin detected. Depends upon helper adenovirus for replication.Temperature: 37°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus and helper virus(e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 1.0 each for virus and helper virus). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 2-3 days at 37°C in a humidified 5% CO2 atmosphere, until helper virus CPE is progressed through 80-90% of the monolayer. AAV does not cause CPE.
Hoggan MD, et al. Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics. Proc. Natl. Acad. Sci. USA 55: 1467-1474, 1966. PubMed: 5227666
