最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > Adeno-associated virus 3 B222891
Adeno-associated virus 3
Adeno-associated virus 3
規格:
貨期:
編號:B222891
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Adeno-associated virus 3
商品貨號
B222891
Classification
Parvoviridae, Dependovirus
Agent
Adeno-associated virus 3
Strain
H
Common Name
AAV-3H
Applications
Cross-reacts with AAV-2; no hemagglutinin detected.
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen
Storage Conditions
?-70°C or colder
Comments
Requires co-infection with helper adenovirus Adenovirus type 2 (ATCC VR-846) for replication. Helper virus may be inactivated after harvest in water bath at 56°C for 30 minutes. Cross-reacts with AAV-2; no hemagglutinin detected.
Effect on Host
AAV produces no CPE. Helper adenovirus induces refractile rounding and cell sloughing.
Recommended Host
Production Host: HEK-293 (ATCC CRL-1573).

Production Host: ?HEK-293 (ATCC? CRL-1573™)

Alternate Hosts: HEK cells, KB cells (ATCC CCL-17) and HeLa cells (ATCC CCL-2)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plant cells 24-48 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with helper adenovirus and a small volume (e.g. 1 mL per 25 cm2) of virus diluted with VGM to provide a MOI of about 0.1-1.0. Adsorb 2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 2-3 days at 37°C temperature in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture.
Name of Depositor
MD Hoggan
Chain of Custody
Deposited as Adeno-associated virus type 3
Source
Adenovirus 7, SV-40 hybrid E 46 plus strain infected HEK cells
References

Hoggan MD, et al. Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics. Proc. Natl. Acad. Sci. USA 55: 1467-1474, 1966. PubMed: 5227666

  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479