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Human echovirus 9

規格:

貨期:

編號:B162945

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

Human echovirus 9

商品貨號

B162945

Classification

Picornaviridae, Enterovirus, Enterovirus B

Deposited As

Echovirus 9

Agent

Human echovirus 9

Strain

Hill

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation

Rectal swab from healthy child, Ohio

Product Format

frozen

Storage Conditions

-70°C or colder (or colder)

Comments

This strain is not pathogenic for newborn mice. Related antigenically to Coxsackievirus Type A23. Wigand, R. et al., Arch. Gesamte Virusforsch. 11: 708, 1962.

Effect on Host

Yes, in vitro effects: Cytopathic effects (refractile rounding and sloughing)

CPE, refractile rounding and sloughing

Growth Medium

Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%

Recommended Host

Recommended Host:? LLC-MK₂ (ATCC CCL-7)

Growth Conditions

Atmosphere: 5% CO2 in air recommended

Temperature: 37.0°C

Duration: 2-5 days; For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent) for inoculation.

Cryopreservation

Storage temperature: -70°C

Name of Depositor

HA Wenner

Source

Rectal swab from healthy child, Ohio

References

Ramos-Alvarez M, Sabin AB. Characteristics of poliomyelitis and other enteric viruses recovered in tissue culture from healthy American children. Proc. Soc. Exp. Biol. Med. 87: 655-661, 1954. PubMed: 13237336

Ramos-Alvarez M, Sabin MB. Intestinal viral flora of healthy children demonstrable by monkey kidney tissue culture. Am. J. Public Health 46: 295-299, 1956. PubMed: 13292578

Wigand R, Sabin AB. Antigenic purity and plaque properties of the prototype strains of ECHO virus types 7 to 11, and 17 and 18. Arch. Gesamte Virusforsch. 11: 708-717, 1962. PubMed: 14040255

or colder

Cross References

Nucleotide (GenBank) : U11710 Echovirus 9 Hill polyprotein gene, 5' UTR.

B244801：Human adenovirus 21

B244635：Human echovirus 31

B244601：Human herpesvirus 5

B244599：DNA from Human herpesvirus 1, strain HF

B244392：Human echovirus 21

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