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Human Coxsackievirus B4

規(guī)格:

貨期:

編號:B162872

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

Human Coxsackievirus B4

商品貨號

B162872

Classification

Picornaviridae, Enterovirus, Enterovirus B

Deposited As

Coxsackievirus B 4

Agent

Human Coxsackievirus B4

Strain

J.V.B. (Benschoten)

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation

Stool from 10-year-old child, New York, 1951

Product Format

frozen 1.0 mL

Storage Conditions

-70°C or colder

Images

Effect on Host

CPE, cell rounding and sloughing

Recommended Host

LLC-MK2 Derivative (ATCC^® CCL-7.1™)

Growth Conditions

Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm²). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO₂ atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-7 days at 37°C in a humidified 5% CO₂ atmosphere, until CPE is progressed through 80% of the monolayer.

Name of Depositor

H Plager

Source

Stool from 10-year-old child, New York, 1951

Year of Origin

1951

References

Dalldorf G. The Coxsackie viruses. Bull NY Acad.. Med 26: 329-335, 1950. PubMed: 15411582

Sickles GM, et al. Isolation and type determination of Coxackie virus, group B, in tissue culture. Proc. Soc. Exp. Biol. Med. 88: 22-24, 1955. PubMed: 14357332

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