Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Temperature: 37°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 90-100% confluent. Pre-treat inoculum in EMEM (ATCC® 30-2003™) + 5 μg/mL 1X-S trypsin (Sigma-Aldrich® T0303) for 30 minutes at 37°C. Remove medium from host cells and rinse twice with 1X DPBS. Inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (0.1 to 1.0). Adsorb 1 hour at 37°C in a humidified 5% CO2 atmosphere. End adsorption by removing inoculum and adding virus growth medium.
Incubation: 1-5 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Lee TW, Kurtz JB. Prevalence of human astrovirus serotypes in the Oxford region 1976-92, with evidence for two new serotypes. Epidemiol Infect. 1112(1): 187-193, 1994. PubMed: 8119358
