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pSCH4500

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價格:

編號:B244876

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

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產品名稱

pSCH4500

商品貨號

B244876

Designations

pSCH4500

GenBank Number

L06156

培養基

Species

Providencia stuartii (Buttiaux et al.) Ewing

Depositors

KJ Shaw

Applications

The 375bp BclI fragment is recommended as a hybridization probe for aac(2')-Ia.

Vector

Construct size (kb): 4.25

Insert

DNA: genomic

Insert lengths(kb): 1.305999994277954

Gene product: aminoglycoside 2'-N-acetyltransferase [aac(2')-Ia]

Insert Size (kb)

1.306

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): EcoRV/XhoI--2.9, 1.3; EcoRI--4.3; XhoI--4.3; EcoRI--4.3; HindIII--uncut; BamHI--4.3.

The insert contains the following restriction sites (approximate kb from the 5' end): HpaI--0.01; BclI--0.41, 0.79; NdeI--0.46; BstXI--0.53.

Two rounds of gel purification of the restriction digest generated probe is necessary. Failure to do so often results in high backgrounds (due to flanking regions in the plasmid insert) and false positives with clinical E. coli strains.

The 375bp BclI fragment is recommended as a hybridization probe for aac(2')-Ia.

References

Shaw KJ, et alThe application of molecular techniques for the study of aminoglycoside resistanceIn: Shaw KJ, et alMethods in molecular medicine: molecular approaches for the diagnosis and investigation of bacterial diseasesTotowa, NJHumana Presssubmitted, 1996

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