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Derivation

NCEB-1 was established by Epstein-Barr virus (EBV) transformation of peripheral blood mononuclear cells from a patient with centroblastic-centrocytic diffuse lymphoma expressing IgM lambda. Cytogenetic analysis of the cell line demonstrated two clones with variations: a hypodiploid clone, with a complex karyotype including a t(11;14)(q13;q32) similar to the original tumor cells, and a near tetraploid clone with the same markers. The cell line was not tumorigenic when tested in an in vitro assay using immunosuppressed mice. [PubMed: 2848599]. It was reported in 2006 that surprisingly, NCEB-1 carried several stable mouse chromosomes and showed expression of both human and murine bcl-2 protein. [PubMed: 16448697]. ATCC cautions scientist on the use of the NCEB-1 cell line as an in vitro model for the study of Mantle Cell Lymphoma. Published data from researchers and testing performed during the ATCC accessioning process have confirmed that this cell line is a human-mouse hybrid. This finding at ATCC is based on karyotyping analysis (G-banding).

Clinical Data

NCEB-1 was established by Epstein-Barr virus (EBV) transformation of peripheral blood mononuclear cells from a patient with centroblastic-centrocytic diffuse lymphoma expressing IgM lambda.

Antigen Expression

CD3-, CD5+, CD10-, CD19+, CD20+, CD23+, FMC7- (verified at ATCC)

Genes Expressed

CD3-, CD5+, CD10-, CD19+, CD20+, CD23+, FMC7- (verified at ATCC)

Comments

NCEB-1 was established by Epstein-Barr virus (EBV) transformation of peripheral blood mononuclear cells from a patient with centroblastic-centrocytic diffuse lymphoma expressing IgM lambda. Cytogenetic analysis of the cell line demonstrated two clones with variations: a hypodiploid clone, with a complex karyotype including a t(11;14)(q13;q32) similar to the original tumor cells, and a near tetraploid clone with the same markers. The cell line was not tumorigenic when tested in an in vitro assay using immunosuppressed mice. [PubMed: 2848599]. It was reported in 2006 that surprisingly, NCEB-1 carried several stable mouse chromosomes and showed expression of both human and murine bcl-2 protein. [PubMed: 16448697]. ATCC cautions scientist on the use of the NCEB-1 cell line as an in vitro model for the study of Mantle Cell Lymphoma. Published data from researchers and testing performed during the ATCC accessioning process have confirmed that this cell line is a human-mouse hybrid. This finding at ATCC is based on karyotyping analysis (G-banding).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Subculturing

Protocol: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(4) to 2 X 10(4) viable cells/ml. Do not allow the cell concentration to exceed 1.2 X 10(6) cells/ml.

Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cryopreservation

Freeze medium: fetal bovine serum, 60%; RPMI-1640 Medium, 30%; DMSO, 10%

strong>Storage temperature: liquid nitrogen vapor phase

STR Profile

Amelogenin: X

CSF1PO: 12,13

D13S317: 12

D16S539: 9,10

D5S818: 9,11

D7S820: 10,11

THO1: 6,9.3

TPOX: 11

vWA: 14,18

Population Doubling Time

about 19 hours

Name of Depositor

E. Campo

Year of Origin

1987

References

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697

Saltman, D.L., et al. Characterization of a new non-Hodgkin's lymphoma cell line (NCEB-1) with a chromosomal (11:14) translocation [t(11:14)(q13;q32)]. Blood.;72(6):2026-2030 (1988). PubMed: 2848599