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3B11
3B11
規格:
價格:
編號:B244534
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
3B11
商品貨號
B244534
Organism
Ictalurus punctatus, channel catfish
Tissue
peripheral blood
培養基
Cell Type
lymphocyte B lymphoblast
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
They are reported to secrete moderate levels of IgM in culture [PubMed: 8133033].
Storage Conditions
liquid nitrogen vapor phase
Karyotype
diploid
Derivation
1G8 (ATCC CRL-2756), developed in 1992, and 3B11 (ATCC CRL-2757), developed in 1994, are immortal B cell lines developed by in vitro lipopolysaccharide (LPS) stimulation of peripheral blood from two different normal channel catfish. The cells were subsequently cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. They are reported to secrete moderate levels of IgM in culture [PubMed: 8133033]. The 3B11 cells constitutively express telomerase [PubMed: 11687262].
Comments
1G8 (ATCC CRL-2756), developed in 1992, and 3B11 (ATCC CRL-2757), developed in 1994, are immortal B cell lines developed by in vitro lipopolysaccharide (LPS) stimulation of peripheral blood from two different normal channel catfish. The cells were subsequently cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. They are reported to secrete moderate levels of IgM in culture [PubMed: 8133033]. The 3B11 cells constitutively express telomerase [PubMed: 11687262].
Complete Growth Medium
A 1:1 mixture of Leibovitz's L-15 medium with 2 mM L-glutamine, and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 87.5%; double distilled water, 10%; heat-inactivated Catfish serum, 2.5%

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml.
Interval: Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 27.0°C
Name of Depositor
NW Miller
Year of Origin
1994
References

Wilson M, et al. A novel chimeric Ig heavy chain from a teleost fish shares similarities to IgD. Proc. Natl. Acad. Sci. USA 94: 4593-4597, 1997. PubMed: 9114035

Stuge TB, et al. Development and analysis of various clonal alloantigen-dependent cytotoxic cell lines from channel catfish. J. Immunol. 164: 2971-2977, 2000. PubMed: 10706684

Barker K, et al. Immortal and mortal clonal lymphocyte lines from channel catfish: comparison of telomere length, telomerase activity, tumor suppressor and heat shock protein expression. Dev. Comp. Immunol. 26: 45-51, 2002. PubMed: 11687262

Miller N, et al. Functional and molecular characterization of teleost leukocytes. Immunol. Rev. 166: 187-197, 1998. PubMed: 9914913

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