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pUR278-183K

規格:

價格:

編號:B243921

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pUR278-183K

商品貨號

B243921

Designations

pUR278-183K

Species

Tobacco mosaic virus

培養基

Depositors

WO Dawson

Applications

produces protein 183K protein

Vector

Construct size (kb): 6.329999923706055

Insert

DNA: cDNA

Insert lengths(kb): 1.129999995231628

Gene product: 183K protein

Target Gene: 183K protein

Insert Size (kb)

1.130

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): PstI--5.6, 0.8; EcoRI--5.6, 0.8, 0.5; HindIII--6.8; BamHI/ClaI--3.3, 2.3, 1.25; BamHI--6.8; ClaI--3.6, 3.2.

This clone expresses an antigen specific for the 183K protein of tobacco mosaic virus.

A fragment containing the 183K gene (nt 3572 to approx. 4700) of pTMV204 was ligated into pUR278.

Classification

Tobamovirus

References

Dawson WO, et al. cDNA cloning of the complete genome of tobacco mosaic virus and production of infectious transcripts. Proc. Natl. Acad. Sci. USA 83: 1832-1836, 1986.

Lehto K, et al. Time course of TMV 30K protein accumulation in intact leaves. Virology 174: 290-293, 1990. PubMed: 2294644

William O Dawson, personal communication

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