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pHFTA plasmid in <em>Escherichia coli</em>

規(guī)格:

價格:

編號:B243143

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

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產(chǎn)品名稱

pHFTA plasmid in Escherichia coli

商品貨號

B243143

Designations

pHFTA plasmid in Escherichia coli

GenBank Number

L10413

培養(yǎng)基

Species

Homo sapiens, human

Depositors

DA Andres

Vector

Total size of construct (kb): 4.6
Vector: pBluescript SK+
Vector size (kb): 2.9
Markers: ampR
Vector ends: EcoRI

Insert

Insert size (kb): 1.67
Gene: farnesyltransferase, CAAX box, alpha, FNTA
DNA: cDNA
Insert ends: EcoRI
Source: human
Genbank accession: L10413

Insert Size (kb)

1.670

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Freeze dried E. coli containing the plasmid

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 1.67; EcoRV--3.9, 0.7; SmaI--4.3, 0.3; HindIII--4.6; PstI--3.1, 0.55, 0.35 (doublet), 0.25.

Transcription from the T3 promoter gives the sense strand.

References

Andres DA, et al. cDNA cloning of the two subunits of human CAAX farnesyltransferase and chromosomal mapping of FNTA and FNTB loci and related sequences. Genomics 18: 105-112, 1993. PubMed: 8276393

Douglas A Andres, personal communication

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