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pBD64

規格:

價格:

編號:B242594

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pBD64

商品貨號

B242594

Designations

pBD64

Depositors

D Dubnau

培養基

Biosafety Level

Vector Information

Size (kb): 4.6999998092651370

Vector: pBD64 (plasmid)

Construction: pUB110, pC194

Marker(s):cmlR,kanR

Construct size (kb): 4.699999809265137

Features: marker(s): cmlR, kanR
replicon: pUB110

Applications

shuttle vector

Comments

A general purpose plasmid vector for cloning in Bacillus subtilis.

This vector was isolated as a spontaneous deletion variant of pBD12, a chimeric vector composed of plasmids pUB110 and pC194. The deletion consists of 1844 bp (nt 3803 to 5649) of pBD12, and encompasses nt 1 to 487 and 1552 to 2906 of the pC194 sequence.

Media

ATCC^® Medium 1179: VY medium

Growth Conditions

Temperature: 37.0°C

References

Gryczan T, et al. Characterization of chimeric plasmid cloning vehicles in Bacillus subtilis. J. Bacteriol. 141: 246-253, 1980. PubMed: 6243622

Minton NP, et al. The gram-positive cloning vector pBD64 arose by a 1844 bp deletion of pC194 derived DNA. Nucleic Acids Res. 18: 1651, 1990. PubMed: 2326208

Shipped

freeze-dried

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