Vector Information
Size (kb): 4.6999998092651370
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: SmaI closer to BamHI site
Vector end: SmaI
Cloning sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I
HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI
SacII BstXI SacI
Polylinker sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I
HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI
SacII BstXI SacI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Vector: pBluc* (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pBluescript SK+, pUHC131-1
Marker(s):ampR
Construct size (kb): 4.699999809265137
Features: insert detection: lacZ'
marker(s): ampR
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: Kpn...SacI
coding sequence: luc*
