最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pSVA3 B241866
pSVA3
pSVA3
規格:
價格:
編號:B241866
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pSVA3
商品貨號
B241866
Designations
pSVA3
Species
Cricetulus griseus, hamster, Chinese
培養基
Depositors
PW Melera
Applications
in another host, produces protein dihydrofolate reductase
Vector
Construct size (kb): 5.099999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSV2-neo
Intact vector size: 5.729
Type of vector: plasmid
Vector end: HindIII
Vector end: SmaI
Cloning sites: EcoRI BamHI PvuII PstI HindIII
Polylinker sites:
Construction: SV40, Tn5, pBR322
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
replicon: SV40
marker(s): G418R, <-
restriction site: HindIII
promoter: SV40 early, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: U02434
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: hamster, Chinese
Gene symbol: DHFR
Gene name: dihydrofolate reductase
Contains complete coding sequence?: U
Tissue: lung cell line
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: BglII
Insert size (kb): 0.71
Cross references:

Insert information:

Insert size (kb): 0.71

DNA : cDNA

Gene : DHFR

Source : hamster, Chinese lung cell line

Insert lengths(kb): 0.7099999785423279
Tissue: lung cell line
Gene product: dihydrofolate reductase [DHFR]
Target Gene: dihydrofolate reductase
Insert Size (kb)
0.710
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.5, 1.7; EcoRI--2.7, 2.3; PstI--1.9, 1.4, 0.95, 0.9; PvuII--4.0, 1.1; HindIII/SmaI--5.2.
Transfected cells amplify DHFR sequences.
Encodes the L22F mutant of DHFR that can serve as a dominant selection marker because it retains catalytic activity and also confers methotrexate (MTX) resistance at low MTX concentrations (125 nM).
Constructed by cloning a HindIII/SmaI fragment containing the gene into pSV2-neo from which neoR had been removed. This plasmid was then cut with SmaI and BglII to remove 18 G nucleotides at the 3' end.
The BglII site was filled in, and ligated to the SmaI site, thereby destroying the original BglII and SmaI sites.
Removing the HindIII/SmaI fragment from pSV2-neo removes all but the 3' 171 bp of neoR.
References

Hussain A, et al. Construction of a dominant selectable marker using a novel dihydrofolate reductase. Gene 112: 179-188, 1992. PubMed: 1555767

  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479