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AKAP100

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編號:B241670

品牌:Mingzhoubio

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RNA

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產品名稱

AKAP100

商品貨號

B241670

Designations

AKAP100

GenBank Number

U17195

培養基

Species

Homo sapiens, human

Depositors

JD Scott

Applications

in suitable host, produces protein A-kinase anchor protein 100

Vector

Construct size (kb): 7.639999866485596

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pET11d-kfc
Intact vector size: 5.800
Type of vector: plasmid
Vector end: NcoI
Vector end: BamHI
Cloning sites: NcoI BamHI
Polylinker sites:
Construction: pET11d
Host range: Escherichia coli
Features (with orientation and position when available):
transcription terminator: T7, <-
other: calmodulin binding domain, <-
restriction site: KpnI
restriction site: BglII
other: factor X unit, <-
restriction site: SalI
other: kemptide unit, <-
restriction site: BamHI
epitope tag: T7-tag coding sequence, <-
restriction site: NcoI
promoter for in vitro transcription: T7, <-
repressor gene: lacI, ->
replicon: pMB1
marker(s): ampR, <-
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: AKAP100*
Gene name: A-kinase anchor protein 100
Contains complete coding sequence?: Y
Tissue: hippocampus
Type of DNA: cDNA
Insert 5' end: NcoI
Insert 3' end: BamHI
Insert size (kb): 1.965
Cross references: DNA Seq. Acc.: U17195
Nucleotides 1-1965 of the insert correspond to
nucleotides 1-1965 of U17195.

Insert lengths(kb): 1.9650000333786

Tissue: hippocampus

Gene product: A-kinase anchor protein 100 [AKAP100*]

Target Gene: A-kinase anchor protein 100

Insert Size (kb)

1.965

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BamHI/NcoI--5.8, 2.0; EcoRI--6.0, 1.8; HindIII--7.0, 0.6; PstI--6.2, 1.6; PvuII--3.0, 2.8, 1.5, 0.3.

Constructed by ligating a PCR product creating an NcoI site on the 5' end to a fragment from a cDNA library.

The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.60; HindIII--1.75; NdeI--0.67; PstI--1.55; PvuII--1.31, 1.62; SacI--0.09.

Transcription from the T7 promoter allows expression of a kfc-fusion protein that can be purified by affinity chromatography on calmodulin-agarose. (kfc refers to kemptide - factor X - calmodulin-binding domains).

References

McCartney S, et al. Cloning and characterization of A-kinase anchor protein 100 (AKAP100). A protein that targets A-kinase to the sarcoplasmic reticulum. J. Biol. Chem. 270: 9327-9333, 1995. PubMed: 7721854

B205865：AKAP100

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