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pSE1111
pSE1111
規格:
價格:
編號:B241410
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pSE1111
商品貨號
B241410
Designations
pSE1111
Species
Saccharomyces cerevisiae Meyen ex E.C. Hansen
培養基
Depositors
SJ Elledge
Applications
in another host, produces protein
Vector
Construct size (kb): 8.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pACT
Intact vector size: 7.650
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BglII BamHI XhoI
Polylinker sites: BglII XhoI BamHI EcoRI BglII
Other unique sites: XbaI HapI ClaI EcoRV
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
replicon: 2 micron
marker(s): LEU2, ->
terminator: ADH
MCS: BglII XhoI ... BglII
other: GAL4 activation domain
promoter for expression: ADH, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiaeCandida robusta
Gene symbol: SNF4
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 0.9
Cross references:
Insert lengths(kb): 0.8999999761581421
Gene product: [SNF4]
Insert Size (kb)
0.900
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
component of:ATCC 87014
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.2, 0.9; EcoRI--3.7, 3.0, 0.5; BglII--7.0, 0.94.
pSE1111 (ATCC 87010) and pSE1112 (ATCC 87011) can be used together as a positive control in 2-hybrid cloning systems.
Expresses SNF4 fused to the GAL4 activation domain.
Classification
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581

Stephen J Elledge, personal communication

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