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pbs/PSP94
pbs/PSP94
規(guī)格:
價格:
編號:B240767
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pbs/PSP94
商品貨號
B240767
Designations
pbs/PSP94
Species
Homo sapiens, human
培養(yǎng)基
Vector
Construct size (kb): 3.799999952316284
Insert
DNA: cDNA
Insert lengths(kb): 0.5400000214576721
Tissue: prostate
Gene product: microseminoprotein, beta-(prostatic secretory protein) [MSMB]
Insert Size (kb)
0.540
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): PstI/SstI--3.25, 0.54; PstI--3.8; SstI--3.7; XhoI--uncut; KpnI/BamHI--3.2, 0.5; RsaI--2.25, 0.72 (doublet); XhoI--1.5, 1.0, 0.8.
The insert contains EcoRII (4), MboI (2), NsiI (2), HinfI (2), XhoII (1), RsaI (1), TaqI (1), AvaII (1), and HaeIII (2) sites.
The insert consists of 483 bp of cDNA, 53 bp of dG/dC, and 17 bp of poly(A).
The insert encodes the complete prepolypeptide of 114 amino acids and includes 11 bp 5' noncoding and about 128 bp 3' noncoding sequence. There is no AATAAA polyadenylation signal although there is a AGTAAA.
Detects human genomic restriction fragments of the following sizes (kb): EcoRI--3.4 (and a weaker band of 3.0 kb that may be artifactual); BamHI--3.1, 0.5; PvuII--2.8, 0.8, 0.75.
References

Mbikay M, et al. Molecular cloning and sequence of the cDNA for a 94-amino-acid seminal plasma protein secreted by the human prostate. DNA 6: 23-29, 1987. PubMed: 3829888

Nolet S, et al. Rapid evolution of prostatic protein PSP94 suggested by sequence divergence between Rhesus monkey and human cDNAs. Genomics 9: 775-777, 1991. PubMed: 2037304

Majambu Mbikay, personal communication

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