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pUMA13

規(guī)格:

價(jià)格:

編號(hào):B240347

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pUMA13

商品貨號(hào)

B240347

Designations

pUMA13

Depositors

UA Heinlein

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 3.0000000000000000

Vector: pUMA13 (plasmid)

Promoters: Promoter for in vitro transcription SP6

Construction: pSP64

Marker(s):ampR

Construct size (kb): 3.0

Features: initiation codon: ATG
marker(s): ampR
promoter for in vitro transcription: SP6
replicon: pMB1
MCS: EcoRI...NotI
restriction site: NcoI
signal peptide coding region: NCAM
stop codons: 3

Applications

construct to generate mRNA that can be expressed in Xenopus oocytes

vector for constructing cDNA libraries

vector permitting RNA synthesis in vitro

vector with sequences for protein export

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0; NotI--3.0; XhoI--3.0.

Vector designed for in vitro transcription of cloned inserts encoding open reading frames. Transcribed RNA encodes the murine NCAM signal sequence for protein export and will contain the authentic 5'-UTR of X. laevis beta globin on the 5' end.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Dommers S, Heinlein UA. Recombinant polypeptides transplanted from pUMA vector-derived cRNAs are translocated through microsomal membranes and exported out of frog oocytes. Biochem. Biophys. Res. Commun. 204: 1346-1351, 1994. PubMed: 7980614

Shipped

freeze-dried

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