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pBCRF1
pBCRF1
規格:
價格:
編號:B239932
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pBCRF1
商品貨號
B239932
Designations
pBCRF1
GenBank Number

V01555

培養基
Species
Human gammaherpesvirus 4 (HHV-4), Epstein-Barr virus
Depositors
DNAX Research Institute of Molecular and Cellular Biology, Inc., KW Moore, DNAX Research Institute of Molecular and Cellular Biology, Inc.
Vector
Name of vector: pcDSRalpha
Construct size (kb): 4.30
Type of vector: plasmid
Construction: pcDV1, HTLV-1 LTR
Host range: vertebrate cells; Escherichia coli

Features (with orientation and position when available):
centromere: c
marker(s): ampR
promoter: SV40/HTLV-1 LTR
replicon: pMB1, SV40
Insert
Genome: Epstein-Barr virus
Gene symbol: BCRF1
Gene name: open reading frame BCRF1
Contains complete coding sequence?: U
Type of DNA: genomic
Insert size (kb): 0.7
Cross references: DNA Seq. Acc.: V01555DNA: genomic
Insert lengths(kb): 0.70
Gene product: open reading frame BCRF1 [BCRF1]
Insert Size (kb)
0.70
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.6, 0.7; EcoRI--3.8, 0.5; XhoI--3.6, 0.7.
EcoRI will also excise the insert.
The vector is modified from pcD, with a promoter constructed from the SV40 early promoter and the R segment and U5 sequence of the long terminal repeat of human T-cell leukemia virus type 1 (HTLV-1 LTR).
Classification
Herpesviridae, Lymphocryptovirus
References

Vieira P, et al. Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading fram BCRFI. Proc. Natl. Acad. Sci. USA 88: 1172-1176, 1991. PubMed: 1847510

Hsu DH, et al. Expression of interleukin-10 activity by Epstein-Barr virus protein BCRF1. Science 250: 830-832, 1990. PubMed: 2173142

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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