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pKG1800

規(guī)格:

價(jià)格:

編號(hào):B239747

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pKG1800

商品貨號(hào)

B239747

Designations

pKG1800

Depositors

M Rosenberg

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 4.9000000953674320

Vector: pKG1800 (plasmid)

Construction: pKO1

Marker(s):ampR

Construct size (kb): 4.900000095367432

Features: insert detection: galK
marker(s): ampR
replicon: pMB1

Applications

terminator-cloning vector

Comments

Insertion of terminator sequences at the cloning sites causes the galK+ phenotype to become galK-. galK+ plasmids are lethal on galETK hosts because of galactose-1-phosphate accumulation, so terminator-containing clones survive in galactose media.

A terminator cloning plasmid vector.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

McKenney K, et al. A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase. Gene Amplif. Anal. 2: 383-415, 1981. PubMed: 6101056

Nierman WCVectors for cloning promoters and terminatorsIn: Nierman WCVectors: a survey of molecular cloning vectors and their usesBostonButterworthpp. 153-177, 1988

Shipped

freeze-dried

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