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pI3

規(guī)格:

價格:

編號:B239530

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pI3

商品貨號

B239530

Designations

pI3

Depositors

KW Minton, C Masters

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 16.0000000000000000

Vector: pI3 (plasmid)

Construction: pKK232-8, pUE10

Marker(s):ampR,cmlR

Construct size (kb): 16.0

Features: marker(s): ampR, cmlR
replicon: pMB1, pUE10

Applications

shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--17.0; EcoRI--14.0, 4.3; HindIII--17.0.

Shuttle vector using ampR selection in E. coli and cmlR selection in Deinococcus radiodurans.

The order of the major features in this plasmid is: EcoRI/MCS/HindIII - pUE10 replicon - pUE10 promoter -> - cmlR -> - pMB1 ori - ampR.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Masters CI, Minton KW. Promoter probe and shuttle plasmids for Deinococcus radiodurans. Plasmid 28: 258-261, 1992. PubMed: 1461940

Shipped

freeze-dried

B219941：pI304

B161746：PI3K Genetic Alteration Cell Panel

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