最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > 28S.3 B239502
28S.3
28S.3
規(guī)格:
價格:
編號:B239502
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
28S.3
商品貨號
B239502
Organism
Ictalurus punctatus, channel catfish
Tissue
peripheral blood
培養(yǎng)基
Cell Type
lymphocyte T lymphoblast
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The cells constitutively produce a supernatant factor(s) with growth promoting activity [PubMed: 9809582].
The 28S.3 cell line, developed in 1996, is an immortal T cell line spontaneously derived from a long term culture of peripheral blood from a normal channel catfish.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
diploid
Derivation
The 28S.3 cell line, developed in 1996, is an immortal T cell line spontaneously derived from a long term culture of peripheral blood from a normal channel catfish. The cells were subsequently cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. The cells are immunoglobulin negative [PubMed: 9809582]. The cells constitutively produce a supernatant factor(s) with growth promoting activity [PubMed: 9809582].
Comments
The 28S.3 cell line, developed in 1996, is an immortal T cell line spontaneously derived from a long term culture of peripheral blood from a normal channel catfish. The cells were subsequently cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. The cells are immunoglobulin negative [PubMed: 9809582]. The cells constitutively produce a supernatant factor(s) with growth promoting activity [PubMed: 9809582].
Complete Growth Medium
A 1:1 mixture of Leibovitz's L-15 medium with 2 mM L-glutamine, and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 87.5%; double distilled water, 10%; heat-inactivated Catfish serum, 2.5%

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml.
Interval: Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 27.0°C
Name of Depositor
NW Miller
Year of Origin
1996
References

Wilson MR, et al. T-cell receptors in channel catfish: structure and expression of TCR alpha and beta genes. Mol. Immunol. 35: 545-557, 1998. PubMed: 9809582

  • 王經(jīng)理
    手機號：17280875617
    QQ：1438578920
  • 胡經(jīng)理
    手機號：13345964880
    QQ：2438244627
  • 周經(jīng)理
    手機號：17757487661
    QQ：1296385441
  • 于經(jīng)理
    手機號：18067160830
    QQ：2088210172
  • 沈經(jīng)理
    手機號：19548299266
    QQ：2662369050
  • 李經(jīng)理
    手機號：13626845108
    QQ：972239479