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pHAF7
pHAF7
規(guī)格:
價(jià)格:
編號(hào):B239439
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pHAF7
商品貨號(hào)
B239439
Designations
pHAF7
GenBank Number

J00077

培養(yǎng)基
Species
Homo sapiens, human
Depositors
T Tamaoki
Vector
Construct size (kb): 5.699999809265137
Insert
DNA: cDNA
Insert lengths(kb): 1.200000047683716
Tissue: embryonal carcinoma
Gene product: alpha-fetoprotein( alpha-1-fetoprotein, alpha-foetoprotein, alpha-fetoglobulin) [AFP]
Alleles: B1, B2, C1, C2, D1, D2
Insert Size (kb)
1.200
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.4 + smaller; HindIII--5.7; EcoRI--5.7; AvaI--5.7; BamHI--5.7.
Insert includes 5' portion of mRNA except the first 55 nucleotides. Encoded are part of the signal peptide, all of domain 1, and part of domain 2 of the protein.
This probe overlaps with pHAF6 (p01088).
Enzyme(s) not detecting polymorphism: BamHI, BclI, BglI, BglII, EcoRI, EcoRV, HaeIII, HgiAI, HincII, HindIII, HinfI, KpnI, MspI, MstII, PvuII, RsaI, SacI, Sau3A, StuI, TaqI, XbaI, XmnI using a panel of 10 unrelated Caucasoids.
References

Morinaga T, et al. Primary structures of human alpha-fetoprotein and its mRNA. Proc. Natl. Acad. Sci. USA 80: 4604-4608, 1983. PubMed: 6192439

Murray JC, et al. Linkage disequilibrium of ALB and AFP RFLPs predicts 5' to 3' orientation. Cytogenet. Cell Genet. 40: 708, 1985.

Murray JC, et al. RFLPs for the human alphafetoprotein (AFP), at 4q11-4q13. Nucleic Acids Res. 13: 6794, 1985. PubMed: 2414731

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