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pJCF62

規格:

價格:

編號:B239254

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pJCF62

商品貨號

B239254

Designations

pJCF62

Depositors

JG Cobley

培養基

Biosafety Level

Vector Information

Size (kb): 12.1000003814697300

Vector: pJCF62 (plasmid)

Construction: pJCF22, pFDA ori

Marker(s):cmlR,kanR

Construct size (kb): 12.10000038146973

Features: marker(s): kanR, cmlR
replicon: pMB1, pFDA

Applications

contains sequence DNA Segment

shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): HindIII--2.55, 2.4, 2.2 (doublet), 2.15, 1.1; BamHI--uncut; ClaI--12.1, ClaI/BamHI--12.1.

Can be mobilized into F. diplosiphon using the conjugative plasmid RP4.

Shuttle vector.

A 2.9 kb BamHI/ClaI fragment from a different construct containing the origin of replication has been sequenced. The BamHI site is not retained in this plasmid.

Constructed by inserting a 8.0 kb ClaI fragment, containing the F. diplosiphon pFDA origin of replication, into the AsuII site of pJCF22.

The order of the major features of the plasmid is: kanR - cmlR - F. diplosiphon pFDA ori - bom - oriV.

Media

ATCC^® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol

Growth Conditions

Temperature: 37.0°C

References

Schaefer MR, et al. Plasmids from two morphologically distinct cyanobacterial strains share a novel replication origin. J. Bacteriol. 175: 5701-5705, 1993. PubMed: 8366056

Cobley JG, et al. Construction of shuttle plasmids which can be efficiently mobilized from Escherichia coli into the chromatically adapting cyanobacterium Fremyella diplosiphon. Plasmid 30: 90-105, 1993. PubMed: 8234495

John G Cobley, personal communication

Shipped

freeze-dried

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