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pBJ5-HD1-F
pBJ5-HD1-F
規(guī)格:
價格:
編號:B239252
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pBJ5-HD1-F
商品貨號
B239252
Designations
pBJ5-HD1-F
GenBank Definition

Human histone deacetylase HD1 mRNA, complete cds

培養(yǎng)基
GenBank Number

U50079

Species
Homo sapiens, human
Depositors
PG Mark
Applications
In another host, produces protein histone deacetylase 1
Vector
Name of construct: pBJ5-HD1-F
Excise insert: BamHI
Promoter giving sense strand: SRalpha
Size of construct (kb): 4.8
Markers: ampR

Name of vector: pBJ5
Intact vector size: 3.300
Type of vector: plasmid
Vector end: SacII
Vector end: EcoRI
Cloning sites: NotI SacII SfiI EcoRI
Polylinker sites: NotI SacII SfiI EcoRI BamHI
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
promoter for expression: SRalpha, →
other: 16S splice site, →
MCS: NotI...EcoRI, →
transcription terminator: polyadenylation, →
marker(s): ampR
Insert
Genome: Human
Gene symbol: HDAC1
Gene name: histone deacetylase 1
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1 p34.1
Tissue: Jurkat T cells
Type of DNA: cDNA
Insert 5' end: NotI, SacII
Insert 3' end: SalI
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: U50079
Nucleotides ~1-1449 of the insert correspond to nucleotides 111-1559 of U50079.
Insert lengths(kb): 1.5
Tissue: Jurkat T cells
Gene product: histone deacetylase 1 [HDAC1]
Target Gene: histone deacetylase 1
Insert Size (kb)
1.5
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.3, 1.5; EcoRV--4.8.
Produces protein tagged at the C-terminus with the FLAG epitope.
References

Taunton J, et al. A Mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p. Science 272: 408-411, 1996. PubMed: 8602529

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