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pG4TH

規格:

價格:

編號:B238983

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pG4TH

商品貨號

B238983

Designations

pG4TH

GenBank Number

J04758

培養基

Species

Mus musculus, mouse

Depositors

D Goldman, DA Nielsen

Vector

Construct size (kb): 4.5

Insert

DNA: cDNA

Insert lengths(kb): 1.728999972343445

Tissue: mastocytoma cell line P815 (ATCC TIB 64)

Gene product: tryptophan hydroxylase (tryptophan 5-monooxygenase) [Tph]

Insert Size (kb)

1.729

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.2, 0.6; BamHI--3.3, 1.3; HindIII--4.6; PstI--3.5, 1.1, EcoRI/BamHI--2.7, 1.2, 0.5 + smaller.

The insert contains the following restriction sites (nt from the 5' end): BamHI--478; EcoRI--595; PstI--673; PvuII--1080.

Detects genomic PstI fragments of the following sizes (kb): mouse--6.8, 4.3; Chinese hamster--5.6, 1.9, 0.9.

Detects mRNA of approximately 1.9 kb (major) and 4.0 kb (minor) in P815 cells.

Transcription from the SP6 promoter gives the sense strand.

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

References

Stoll J, et al. Characterization and chromosomal mapping of cDNA encoding tryptophan hydroxylase from a mouse mastocytoma cell line. Genomics 7: 88-96, 1990. PubMed: 2110547

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