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pNRSac [p Sac]

規格:

價格:

編號:B238982

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pNRSac [p Sac]

商品貨號

B238982

Designations

pNRSac [p Sac]

Species

Homo sapiens, human

培養基

Depositors

RA Weinberg

Applications

The recommended probe is the 0.76 kb EcoRI/HindIII exon 2 fragment.

Vector

Construct size (kb): 9.0

Insert

DNA: genomic

Insert lengths(kb): 7.0

Tissue: HL-60 cell line (promyelocytic leukemia)

Gene product: neuroblastoma RAS viral (v-ras) oncogene homolog( v-ras neuroblastoma RAS viral oncogene homolog, proto-oncogene N-ras) [NRAS]

Alleles: A1, A2

Insert Size (kb)

7.0

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--5.5, 3.5; PvuII--4.2, 3.4, 1.0, 0.6.

The insert can transform NIH 3T3 cells. The recommended probe is the 0.76 kb EcoRI/HindIII exon 2 fragment.

Contains pBR322 (EcoRI/PvuII, 2.05 kb); SV40 ori (PvuII/HindIII, 344 bp); exon 1 (HindIII/HindIII); exon 2 (HindIII/EcoRI, 760 bp); exon 3/exon 4 (EcoRI/SacI, 5 kb), and SV40 polyadenylation signal (SacI/EcoRI).

References

Murray MJ, et al. The HL-60 transforming sequence: a ras oncogene coexisting with altered myc genes in hematopoietic tumors. Cell 33: 749-757, 1983. PubMed: 6683594

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