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pYC7

規格:

價格:

編號:B238622

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pYC7

商品貨號

B238622

Designations

pYC7

Depositors

DJ Stillman

培養基

Biosafety Level

Vector Information

Size (kb): 10.0600004196167000

Vector: pYC7 (plasmid)

Promoters: Promoter truncated CYC1 promoter (no UAS element)

Construction: pBR322

Marker(s):URA3,ampR

Construct size (kb): 10.0600004196167

Features: marker(s): URA3
marker(s): ampR
promoter: truncated CYC1 promoter (no UAS element)
replicon: 2 micron ori
replicon: pMB1
coding sequence: lacZ

Applications

YE-type (episomal) shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): KpnI--10.3; HindIII--8.2, 2.25; BglII--10.3.

This is one of three lacZ reporter plasmids that lack UAS promoter elements (ATCC 87509, 87510 & 87513). They differ by polylinker sites.

DNA fragments can be inserted into these plasmids to assay UAS (upstream activation site) activity.

The vector was constructed by William Timberlake.

Growth Conditions

Temperature: 37.0°C

References

Chang YC, Timberlake WE. Identification of Aspergillus brlA responsive elements (BREs) by genetic selection in yeast. Genetics 133: 29-38, 1993. PubMed: 8417986