Comments
pSM6 can be maintained in both E. coli and B. subtilis but can be used as a cloning vector only in E. coli because the tetracycline resistance is poorly expressed in B. subtilis.
Constructed by ligating pBR322 and pE194 after linearizing with PstI. The EcoRI site was destroyed and a HpaI/SstI fragment containing ErmC was removed. The ErmC promoter and ribosome binding sequence was inserted upstream of an EcoRI linker.
The EcoRI cloning site is 4 bp downstream of the ribosome binding sequence.
