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Myf-5/18-7
Myf-5/18-7
規格:
價格:
編號:B238527
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Myf-5/18-7
商品貨號
B238527
Designations
Myf-5/18-7
Species
Homo sapiens, human
培養基
Applications
The 3' XhoII/EcoRI fragment has been used as a specific probe.
A 750 bp PstI/PvuII fragment, which does not contain the myc homology region of Myf5, was used to identify murine Myf-5 and herculin clones from a genomic library.
Vector
Construct size (kb): 4.5
Insert
DNA: cDNA
Insert lengths(kb): 1.259999990463257
Tissue: fetal skeletal muscle
Gene product: myogenic factor 5 [MYF5]
Insert Size (kb)
1.260
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Insert contains the following sites separated by (bp)(approx): EcoRI/PstI- 50- HaeII- 100- HaeII- 15- HincII- 70- XhoII- 15- FokI/PstI- 265- XhoII- 35- HinfI- 400- HinfI/PvuII- 310- EcoRI.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.6, 1.2; HincII--4.2, 0.26; PvuII--2.7, 1.3, 0.36; SphI--3.8, 0.41; HindIII--4.5.
This is a shorter version of Myf-5 which extends from the PstI site at nucleotide 185 to 1430. It does not include the complete coding sequence, which extends from nucleotide 43 through 807.
Shows weak cross-hybridization to the mouse MyoD1 probe. Amino acid sequence has a region highly similar to myc proteins and the Drosophila developmental factors achaete scute and twist.
The 3' XhoII/EcoRI fragment has been used as a specific probe.
A 750 bp PstI/PvuII fragment, which does not contain the myc homology region of Myf5, was used to identify murine Myf-5 and herculin clones from a genomic library.
References

Braun T, et al. A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts. EMBO J. 8: 701-709, 1989. PubMed: 2721498

Braun T, et al. Differential expression of myogenic determination genes in muscle cells: possible autoactivation by the Myf gene products. EMBO J. 8: 3617-3625, 1989. PubMed: 2583111

Miner JH, Wold B. Herculin, a fourth member of the MyoD family of myogenic regulator genes. Proc. Natl. Acad. Sci. USA 87: 1089-1093, 1990. PubMed: 2300571

Hans H Arnold, personal communication

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