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pKLAf1IIRM520-4

規格:

價格:

編號:B238513

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pKLAf1IIRM520-4

商品貨號

B238513

Designations

pKLAf1IIRM520-4

Species

Anabaena flos-aquae (Lyngbye) Brebisson, blue-green algae

培養基

Depositors

New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc.

Vector

Construct size (kb): 16.20

Insert

DNA: genomic

Insert lengths(kb): 12.4

Gene product: modification methylase AflII

Gene product: restriction endonuclease AflII

Insert Size (kb)

12.400

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--11.5, 3.4, 2.3; BamHI--17.0; HindIII--7.8, 4.4, 2.5, 1.1, 0.4; PstI--12.0, 4.7.

The insert contains the following restriction sites (approximate kb from the 5' XhoII site): EcoRI--2.8, 5.0; HindIII--8.1, 10.6, 11.05, 12.05; NsiI--8.0; PstI--1.8; EcoRV--5.2; NcoI--11.8; SpeI--2.9, 5.5, 9.7; XhaII--2.1.

Distributed in aliquots of 250 ng (10 ng/ul).

References

Lunnen KD, Wilson GG. Method for producing the AflII restriction endonuclease and methylase. US Patent 5,030,569 dated Jul 9 1991

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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