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pWHV8 plasmid in <em>Escherichia coli</em>

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編號:B238296

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

平板

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產品名稱

pWHV8 plasmid in Escherichia coli

商品貨號

B238296

Designations

pWHV8 plasmid in Escherichia coli

GenBank Definition

Woodchuck hepatitis B virus (WHV8), complete genome

培養基

GenBank Number

J04514

Species

Woodchuck hepatitis virus, Woodchuck hepatitis virus

Depositors

Roger Miller

Vector

Construct size (kb): 9.4

Vector: pBR325

Vector size (kb): 6.0

Insert

Insert: Woodchuck hepatitis virus (number 8)

insert size (kb): 3.23

Insert ends: EcoRI

DNA: genomic

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Related Products

Purified plasmid DNA: ATCC 45097D

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

This clone contains the complete viral genome of the woodchuck hepatitis virus cloned into the pBR325 plasmid vector. The virus was linearized at the EcoRI site and inserted into the EcoRI site of pBR325. The EcoRI site interrupts the pre-surface coding region.

Restriction digests of the clone give the following sizes (kb): EcoRI - 6.0, 3.3; BamHI - 7.7, 1.6; PstI - 5.1, 4.1; HindIII - 7.0, 2.3; SalI - 9.4. ---ATCC staff

Classification

Hepadnaviridae, Orthohepadnavirus

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