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P-6
P-6
規(guī)格:
價格:
編號:B238020
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
P-6
商品貨號
B238020
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody can be used for immunochemical and Western blot applications.
It is a useful tool for detecting and localizing the large subunit of calpain.
The antibody is specific for the large subunit of human mu-calpain and shows no cross-reactivity with m-calpain [PubMed: 1426051].
Storage Conditions
liquid nitrogen vapor phase
Derivation
Animals were immunized with mu-calpain isolated from human erythrocytes [PubMed: 1426051]. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody is specific for the large subunit of human mu-calpain and shows no cross-reactivity with m-calpain [PubMed: 1426051]. The antibody can be used for immunochemical and Western blot applications. It is a useful tool for detecting and localizing the large subunit of calpain. A culture submitted to the ATCC in April of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.
Comments
Animals were immunized with mu-calpain isolated from human erythrocytes [PubMed: 1426051]. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody is specific for the large subunit of human mu-calpain and shows no cross-reactivity with m-calpain [PubMed: 1426051]. The antibody can be used for immunochemical and Western blot applications. It is a useful tool for detecting and localizing the large subunit of calpain. A culture submitted to the ATCC in April of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) viable cells/ml.;
Interval: Maintain cell density between 2 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
mouse IgG1 kappa
Name of Depositor
R Lane, RL Mellgren
Deposited As
mouse (B cell); mouse (myeloma)
Year of Origin
1990
References

Lane RD, Mellgren RL. A comparison of the intracellular distribution of mu-calpain, m-calpain, and calpastatin in proliferating human A431 cells. Exp. Cell Res. 203: 5-16, 1992. PubMed: 1426051

Mice were immunized with mu-calpain isolated from human erythrocytes.

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