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pCMV-Myc-S1P

規(guī)格:

價格:

編號:B237963

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pCMV-Myc-S1P

商品貨號

B237963

Designations

pCMV-Myc-S1P

GenBank Number

AF078105

培養(yǎng)基

Species

Cricetus cricetus, hamster

Depositors

J Sakai, RB Rawson

Applications

in another host, produces protein sterol-regulated site-1 protease

Vector

Construct size (kb): 10.19999980926514

Insert

DNA: cDNA

Insert Information:

Insert size (kb): 4.4

Genome: Hamster

Gene symbol: S1P

Gene name: sterol-regulated site-1 protease

Type of DNA: cDNA

Genbank Accession: AF078105

Insert lengths(kb): 4.400000095367432

Gene product: sterol-regulated site-1 protease [S1P]

Target Gene: sterol-regulated site-1 protease

Insert Size (kb)

4.400

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

?Frozen glycerol stock of Escherichia coli HB101 containing the plasmid.

Comments

Restriction digests of the clone give the following sizes (kb): BamHI/XbaI -- 5.8, 4.4; EcoRI--9.7, 0.5; HindIII--6.0, 2.4, 1.8.

Insert encodes an epitope-tagged version of the protein including aa 1-23, three novel amino acids (GGR), three tandem copies of the 9E10 human c-Myc epitop tag, followed by aa 24 - 1052 of the protein.

References

Sakai J, et al. Molecular identification of sterol-regulated luminal protease that cleaves SREBPs and controls lipid composition of animal cells. Mol. Cell 2: 505-514, 1998. PubMed: 9809072

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