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pCLL4

規格:

價格:

編號:B237921

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pCLL4

商品貨號

B237921

Designations

pCLL4

Depositors

American Cyanamid Co., JS Feitelson, American Cyanamid Co.

培養基

U.S. Patent

5,002,891

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Biosafety Level

Vector Information

Size (kb): 10.36

Vector: pCLL4 (plasmid)

Construction: pAkij1, pIJ39

Marker(s):ampR,tsrR

Construct size (kb): 10.36

Features: marker(s): ampR
marker(s): tsrR
replicon: pAkij1

Applications

shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): HindIII--9.0, 2.1; PstI--10.5; KpnI--10.5.

Cloning into the PstI site inactivates the ampicillin marker used for selection in Escherichia coli.

The pAkij1 ori may be useful in other taxa such as Bacillus, Staphylococcus and other Actinomycetes, including Streptosporangium, Actinoplanes, Nocardia, and Micromonospora.

Recommendation for verification: HindIII--8.37, 1.99; PstI--10.36; KpnI--9.47, 0.89

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Feitelson JS. Multifunctional plasmid vectors from Actinomadura and Escherichia coli. US Patent 5,002,891 dated Mar 26 1991

Feitelson JS, et al. Genetic and biochemical characterization of the red gene cluster of streptomyces coelicolor A3(2). J. Gen. Microbiol. 131: 2431-2441, 1985. PubMed: 2999302

Shipped

freeze-dried

Shipping Information

Shipped: ?Freeze dried E. coli containing the plasmid

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