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當(dāng)前位置: 首頁 > ATCC代理 > phPAH247 [PAH247] B237659
phPAH247 [PAH247]
phPAH247 [PAH247]
規(guī)格:
價(jià)格:
編號(hào):B237659
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
phPAH247 [PAH247]
商品貨號(hào)
B237659
Designations
phPAH247 [PAH247]
GenBank Number

K03020

培養(yǎng)基
Species
Homo sapiens, human
Vector
Construct size (kb): 6.800000190734863
Insert
DNA: cDNA
Insert lengths(kb): 2.400000095367432
Tissue: liver
Gene product: phenylalanine hydroxylase [PAH]
Alleles: A1, A2, E1, G1, G2, K1, K2, C1, C2, C3, D1, D2, E2, F2, J1, J2, F1, A2
Insert Size (kb)
2.400
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 2.4; HindIII--5.9, 0.65; PvuII--3.6, 3.2; BamHI--5.1, 1.7; EcoRV--6.8.
Restriction sites (with nucleotide positions relative to the published sequence) include: BamHI--1038; BglII--511; HindIII--1311, 1809; PstI--928; PvuII--902, 1610; TaqI--749; XbaI--423; XhoI--748.
The following restriction enzymes do not cut the insert: AatII, AhaII, ApaI, BclI, BglI, BstEII, BstXI, BvuI, ClaI, EcoRI, EcoRV, HaeII, HgaI, KpnI, MluI, MstI, NarI, NruI, PvuI, SacI, SacII, SalI, SfiI, SphI, SstI, SstII, StuI, TthI, XmaIII, and XmnI.
This insert contains a complete coding sequence with translation initiating at nucleotide 223 and ending at nucleotide 1579.
The allele frequencies for the MspI, EcoRI, BglII, PvuII, EcoRV, HindIII, and XmnI are markedly different between Caucasian and Japanese populations.
References

Kwok SC, et al. Nucleotide sequence of a full-length complementary cDNA clone and amino acid sequence of human phenylalanine hydroxylase. Biochemistry 24: 556-561, 1985. PubMed: 2986678

Threadgill DW, et al. Syntenic conservation between humans and cattle: II. human chromosome 12. Genomics 8: 29-34, 1990. PubMed: 2081597

Detera-Wadleigh SD, et al. A TthIII1 polymorphism on the 5' flanking region of the glucocorticoid receptor gene (GRL). Nucleic Acids Res. 19: 1960, 1991. PubMed: 1674374

Savio L Woo, personal communication

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