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pLV57

規格:

價格:

編號:B237388

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pLV57

商品貨號

B237388

Designations

pLV57

Depositors

D O'Connor

培養基

Biosafety Level

Vector Information

Size (kb): 6.0999999046325680

Vector: pLV57 (plasmid)

Construction: pBR325, NTP14m07(ts)

Marker(s):ampR,cmlR,EcoRIm(ts)r+

Construct size (kb): 6.099999904632568

Features: insert detection: R+Mts
marker(s): ampR, cmlR, EcoRIm(ts)r+
replicon: pMB1

Applications

vector permitting positive selection for inserts

Comments

Restriction digests of the clone give the following sizes (kb): HincII--6.2; HindIII/BamHI--6.2; BamHI--uncut.

Positive selection cloning vector employing EcoRI restriction-modification system.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 30.0°C

References

O'Connor CD, Humphreys GO. Expression of the EcoRI restriction-modification system and the construction of positive-selection cloning vectors. Gene 20: 219-229, 1982. PubMed: 6299889

Shipped

freeze-dried

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