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pYNZ32 [RW-10]

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價格:

編號:B237290

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pYNZ32 [RW-10]

商品貨號

B237290

Designations

pYNZ32 [RW-10]

GenBank Definition

Homo sapiens intergenic locus pYNZ32 variable number tandem repeat (VNTR) sequence associated with Huntington disease gene.

培養基

GenBank Number

M62748

M62750

Species

Homo sapiens, human

Depositors

RL White, Y Nakamura

Applications

RFLPs observed under normal stringency of hybridization and washing.

Vector

Construct size (kb): 6.699999809265137

Insert

DNA: genomic

Insert lengths(kb): 4.0

Gene product: DNA Segment, single copy [D4S125]

Alleles: B1, B2

Insert Size (kb)

4.0

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): PstI/EcoRI--3.0, 2.7, 1.0; EcoRI--6.7; EcoRI/HindIII--3.4, 2.7, 0.6; BamHI/HindIII--3.4, 2.7, 0.6.

Shows linkage to D4S10 with a LOD score greater than 20.

RFLPs observed under normal stringency of hybridization and washing.

References

Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pYNZ32) on chromosome 4p [D4S125]. Nucleic Acids Res. 16: 4186, 1988. PubMed: 2897672

Yusuke Nakamura, personal communication

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